A Plastid RNA Polymerase-Associated Protein Is Involved in Early Chloroplast Development in Rice
Shuang Song,
Ying Wang,
Xin Ding,
Yunlu Tian,
Zewan Wu,
Hang Li,
Qing Li,
Yunpeng Wang,
Shirong Zhou,
Xiaoou Dong,
Jianmin Wan,
Linglong Liu
Affiliations
Shuang Song
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Ying Wang
Vegetable Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310022, China
Xin Ding
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Yunlu Tian
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Zewan Wu
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Hang Li
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Qing Li
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Yunpeng Wang
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Shirong Zhou
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Xiaoou Dong
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Jianmin Wan
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Linglong Liu
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Plant Gene Engineering Research Center, Jiangsu Plant Gene Editing Engineer Research Center, National Field Scientific Observation and Research Station of Rice Germplasm Resources in Nanjing, Jiangsu, Sanya Research Institute, Nanjing Agricultural University, Nanjing 210095, China
Plastid-encoded RNA polymerase (PEP) regulates the expression of chloroplast genes involved in photosynthesis and chloroplast development in rice. The PEP-associated protein (PAP) PAP7/pTAC14 is essential for the formation of the PEP complex. However, the function of PAP7 in chloroplast development in rice remains unclear. In this study, we identified a mutant, w81, which displays a yellow-green leaf symptom before the four-leaf stage. The seedlings of the w81 mutant display reduced chlorophyll content, abnormal chloroplast structure, and elevated reactive oxygen species (ROS) level. After the four-leaf stage, plant leaves of the w81 mutant gradually turn green with increased chlorophyll content. Map-based cloning reveals that the PAP7 in the w81 mutant harbors a T to A single-base substitution. This mutation blocks the normal splicing of the fifth intron and generates 74 bp longer transcripts in the mutant. The OsPAP7 protein mainly localizes to the chloroplast and directly interacts with OsPAP5. Our results highlight that OsPAP7 regulates the expression of PEP-dependent chloroplast genes and plays a key role in chloroplast development in rice.
