Assessment of retina electrogenesis in ultraviolet spectrofluorimetry of the eye

Abstract

Purpose was to study how ultraviolet (UV) light (365 nm) applied in low doses (1200 J·m-2), corresponding to the regimens of spectrofluorimetric analysis of the limbus and lens area, to the anterior eye chamber, affects bioelectric activity of the retina. Material and methods. A group of 25 volunteers (12 men and 13 women aged 21.0 ± 1.3 years) with no signs of ophthalmological or somatic pathology underwent spectrofluorimetric assessment of the limbus and lens area, followed by an evaluation of retina electrogenesis state. Results. We revealed no changes in the electric activity of the retina during the whole period of observation. The amplitude of A-wave varied from 75.2 mcV to 86.2 mcV both before and after the spectrofluorimetric examination. There were no significant changes in b-wave characteristics at any periods of investigation; b/a index did not demonstrate any difference from the baseline level. Conclusion. UV radiation (365 nm) of the anterior eye chamber in doses not exceeding those permitted by the SANPIN 5804-91 standard has no effect on the retinal function, should be considered safe, and can be used in future studies.

Keywords

• флуоресценция
• электроретинография
• сетчатка