Infectious Agents and Cancer (Mar 2022)

Prevalence of human papilloma virus (HPV) genotypes between outpatients males and females referred to seven laboratories in Tehran, Iran

  • Iman Rezaee Azhar,
  • Mahmood Yaghoobi,
  • Mir Majid Mossalaeie,
  • Abolghasem Kollaee Darabi,
  • Amir Houshang Nejadeh,
  • Mahbobeh Jamshidi,
  • Ali Ahani,
  • Masoud Karkhane Mahmoodi,
  • Leila Ghalichi,
  • Ayda Shabanzadeh,
  • Angila Ataei-Pirkooh,
  • Arezoo Marjani,
  • Azam Khamseh,
  • Motahareh Shafiei,
  • Parastoo Hosseini,
  • Saber Soltani,
  • Milad Zandi,
  • Parsa Ghafari,
  • Amir Aboofazeli,
  • Azam Ghaziasadi,
  • Seyed Mohammad Jazayeri

DOI
https://doi.org/10.1186/s13027-022-00421-7
Journal volume & issue
Vol. 17, no. 1
pp. 1 – 8

Abstract

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Abstract Background Human papilloma virus (HPV) causes the most common sexually-transmitted infection especially among sexually-active individuals. The aim of study was to characterize the molecular characterization of HPV genotypes between 5176 female and male patients. Methods HPV DNA was extracted from genital swabs of the study participants and amplified by Real Time Polymerase Chain Reaction (PCR). Genotyping was performed for 2525 cases using REALQUALITY RQ-Multi HPV Detection Kit for the identification of 14 high risk (HR) and 2 low risk (LR) HPV genotypes. Demographic figures were analyzed in correlation with virological data statistically. Results Out of 5176 cases from 7 laboratories, 2727 (53%) were positive for HPV, of which. 2372(87%) women and 355 (13%) men were HPV positive. However, in an intra-gender analysis, positive rate was higher in men (355/637, 55.7%) than in women (2372/4539, 52%; P value 0.007). HPV positive patients were younger than negative individuals. Positive rate was higher among age categories 20–40. Genotyping was performed for 2525 cases. Out of 1219 (48%) patients who contained single genotypes, 566 (22%) and 653 (26%) harboured HR and LR genotypes, respectively. In females and males, 1189 (54%) and 117 (37%) contained multiple genotypes. No substantial associations were found between different age categories and HR/LR and multiple genotypes distribution. Conclusion The prevalence of HPV infection in both genders was high. However, men had a higher rate of infection. These observations highlighted the necessity for a plan for targeted education to younger population in the society as well as application of infection control measures against HPV infection, especially in terms of general population mass HPV vaccination.

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