Effect of low temperature on profilins and ADFs transcription and actin cytoskeleton reorganization in Arabidopsis

Abstract

In the present study, we found that the expression patterns of the vegetative profilins and actin depolymerizing factors (ADFs) were specifically altered under low temperature (17 °C) stress using a real-time PCR. Our results show that also reorganization of the actin cytoskeleton was triggered by a low temperature. Facilitation of microfilament (MF) assembly by phalloidin treatment resulted in an enhanced low temperature stress tolerance, whereas blocking MF assembly with latrunculin B resulted in enhanced low temperature stress sensitivity. Our results show that the specific members of the vegetative profilins and ADFs might participate in regulating the response of plants to a low temperature stress, and the actin cytoskeleton is vital for the tolerance of Arabidopsis seedlings to low temperature stress.

Keywords

• latrunculin b
• microfilaments
• phalloidin
• stress tolerance