Frontiers in Oncology (Jun 2020)
Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood
- L. G. Rikkert,
- L. G. Rikkert,
- L. G. Rikkert,
- P. Beekman,
- P. Beekman,
- P. Beekman,
- J. Caro,
- F. A. W. Coumans,
- F. A. W. Coumans,
- A. Enciso-Martinez,
- G. Jenster,
- S. Le Gac,
- W. Lee,
- T. G. van Leeuwen,
- T. G. van Leeuwen,
- G. B. Loozen,
- A. Nanou,
- R. Nieuwland,
- R. Nieuwland,
- H. L. Offerhaus,
- C. Otto,
- D. M. Pegtel,
- M. C. Piontek,
- E. van der Pol,
- E. van der Pol,
- E. van der Pol,
- L. de Rond,
- L. de Rond,
- L. de Rond,
- W. H. Roos,
- R. B. M. Schasfoort,
- M. H. M. Wauben,
- H. Zuilhof,
- H. Zuilhof,
- L. W. M. M. Terstappen
Affiliations
- L. G. Rikkert
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- L. G. Rikkert
- Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- L. G. Rikkert
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- P. Beekman
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- P. Beekman
- Laboratory of Organic Chemistry, Wageningen University, Wageningen, Netherlands
- P. Beekman
- Applied Microfluidics for Bioengineering Research, University of Twente, Enschede, Netherlands
- J. Caro
- Department of Imaging Physics, Delft University of Technology, Delft, Netherlands
- F. A. W. Coumans
- Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- F. A. W. Coumans
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- A. Enciso-Martinez
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- G. Jenster
- Department of Urology, Erasmus University Medical Center, Rotterdam, Netherlands
- S. Le Gac
- Applied Microfluidics for Bioengineering Research, University of Twente, Enschede, Netherlands
- W. Lee
- Optical Sciences Group, Department of Science and Technology, University of Twente, Enschede, Netherlands
- T. G. van Leeuwen
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- T. G. van Leeuwen
- Biomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- G. B. Loozen
- Department of Imaging Physics, Delft University of Technology, Delft, Netherlands
- A. Nanou
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- R. Nieuwland
- Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- R. Nieuwland
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- H. L. Offerhaus
- Optical Sciences Group, Department of Science and Technology, University of Twente, Enschede, Netherlands
- C. Otto
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- D. M. Pegtel
- 0Department of Pathology, Amsterdam UMC, VU University Amsterdam, Amsterdam, Netherlands
- M. C. Piontek
- 1Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, Netherlands
- E. van der Pol
- Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- E. van der Pol
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- E. van der Pol
- Biomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- L. de Rond
- Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- L. de Rond
- Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- L. de Rond
- Biomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
- W. H. Roos
- 1Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, Netherlands
- R. B. M. Schasfoort
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- M. H. M. Wauben
- 2Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands
- H. Zuilhof
- Laboratory of Organic Chemistry, Wageningen University, Wageningen, Netherlands
- H. Zuilhof
- 3School of Pharmaceutical Sciences and Technology, Tianjin University, Tianjin, China
- L. W. M. M. Terstappen
- Department of Medical Cell Biophysics, University of Twente, Enschede, Netherlands
- DOI
- https://doi.org/10.3389/fonc.2020.00608
- Journal volume & issue
-
Vol. 10
Abstract
Extracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1 μm) in blood from cancer patients are associated with poor outcome, and changes in their number can be used to monitor therapy effectiveness. Whereas, small tumor-derived EVs (<1 μm) are likely to outnumber their larger counterparts, thereby offering better statistical significance, identification and quantification of small tdEVs are more challenging. In the blood of cancer patients, a subpopulation of EVs originate from tumor cells, but these EVs are outnumbered by non-EV particles and EVs from other origin. In the Dutch NWO Perspectief Cancer-ID program, we developed and evaluated detection and characterization techniques to distinguish EVs from non-EV particles and other EVs. Despite low signal amplitudes, we identified characteristics of these small tdEVs that may enable the enumeration of small tdEVs and extract relevant information. The insights obtained from Cancer-ID can help to explore the full potential of tdEVs in the clinic.
Keywords
- atomic force microscopy
- electrochemistry
- electron microscopy
- extracellular vesicles
- flow cytometry
- fluorescence microscopy
