Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses

Abstract

Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat–gag (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.

Keywords

• srlv
• genotypes
• nested real-time pcr
• elisa genotyping
• serotyping