A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media
Oleksandra Vozniuk,
Dr. Zdeněk Kejík,
Kateřina Veselá,
Markéta Skaličková,
Petr Novotný,
Róbert Hromádka,
Dr. Jan Hajduch,
Prof. Pavel Martásek,
Prof. Milan Jakubek
Affiliations
Oleksandra Vozniuk
BIOCEV First Faculty of Medicine Charles University 252 20 Vestec Czech Republic
Dr. Zdeněk Kejík
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Kateřina Veselá
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Markéta Skaličková
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Petr Novotný
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Róbert Hromádka
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Dr. Jan Hajduch
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Prof. Pavel Martásek
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Prof. Milan Jakubek
Department of Paediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague Ke Karlovu 455/2 128 08 Prague Czech Republic
Abstract A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3–100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.
