Enhanced mRNA FISH with compact quantum dots

Yang Liu; Phuong Le; Sung Jun Lim; Liang Ma; Suresh Sarkar; Zhiyuan Han; Stephen J. Murphy; Farhad Kosari; George Vasmatzis; John C. Cheville; Andrew M. Smith

doi:10.1038/s41467-018-06740-x

Nature Communications (Oct 2018)

Enhanced mRNA FISH with compact quantum dots

Yang Liu,
Phuong Le,
Sung Jun Lim,
Liang Ma,
Suresh Sarkar,
Zhiyuan Han,
Stephen J. Murphy,
Farhad Kosari,
George Vasmatzis,
John C. Cheville,
Andrew M. Smith

Affiliations

Yang Liu: Department of Bioengineering, University of Illinois at Urbana-Champaign
Phuong Le: Department of Bioengineering, University of Illinois at Urbana-Champaign
Sung Jun Lim: Department of Bioengineering, University of Illinois at Urbana-Champaign
Liang Ma: Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign
Suresh Sarkar: Department of Bioengineering, University of Illinois at Urbana-Champaign
Zhiyuan Han: Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign
Stephen J. Murphy: Biomarker Discovery Program, Center of Individualized Medicine, Mayo Clinic
Farhad Kosari: Biomarker Discovery Program, Center of Individualized Medicine, Mayo Clinic
George Vasmatzis: Biomarker Discovery Program, Center of Individualized Medicine, Mayo Clinic
John C. Cheville: Biomarker Discovery Program, Center of Individualized Medicine, Mayo Clinic
Andrew M. Smith: Department of Bioengineering, University of Illinois at Urbana-Champaign

DOI: https://doi.org/10.1038/s41467-018-06740-x
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

Read online

FISH-based techniques to image and count mRNA in single cells can be limited by the photophysical properties of organic dyes. Here the authors develop photostable quantum dot FISH probes for multiplexed imaging.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal