Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning
Nobuyuki Tanaka,
Ryoji Sekine,
Shun-ichi Funano,
Asako Sato,
Núria Taberner Carretero,
Miki Ebisuya,
Yo Tanaka
Affiliations
Nobuyuki Tanaka
1Laboratory for Integrated Biodevice, Center for Biosystems Dynamics Research, RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Ryoji Sekine
2Laboratory for Reconstitutive Developmental Biology, Center for Biosystems Dynamics Research, RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan
Shun-ichi Funano
1Laboratory for Integrated Biodevice, Center for Biosystems Dynamics Research, RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Asako Sato
1Laboratory for Integrated Biodevice, Center for Biosystems Dynamics Research, RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Núria Taberner Carretero
2Laboratory for Reconstitutive Developmental Biology, Center for Biosystems Dynamics Research, RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan
Miki Ebisuya
2Laboratory for Reconstitutive Developmental Biology, Center for Biosystems Dynamics Research, RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan
Yo Tanaka
1Laboratory for Integrated Biodevice, Center for Biosystems Dynamics Research, RIKEN, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.
