Unusual Bicyclo[3.2.1]Octanoid Neolignans from Leaves of <i>Piper crocatum</i> and Their Effect on Pyruvate Dehydrogenase Activity
Yu Juan Chai,
Younghoon Go,
Hai Qi Zhou,
Hong Xu Li,
Sun Joo Lee,
Yeo Jin Park,
Wahyu Widowatib,
Rizal Rizal,
Young Ho Kim,
Seo Young Yang,
Wei Li
Affiliations
Yu Juan Chai
Health Science Center, School of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China
Younghoon Go
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea
Hai Qi Zhou
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
Hong Xu Li
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
Sun Joo Lee
New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, 80 Cheombok-ro, Dong-gu, Daegu 41061, Korea
Yeo Jin Park
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea
Wahyu Widowatib
Medical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia
Rizal Rizal
Biomolecular Biomedical Research Center for Biology, Indoensian Institute of Sciences, Bandung 40163, Indonesia
Young Ho Kim
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea
Seo Young Yang
Department of Pharmaceutical Engineering, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Korea
Wei Li
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea
Three undescribed bicyclo[3.2.1]octanoid neolignan glucosides, along with a known neolignan, were isolated from the leaves of Piper crocatum Ruiz & Pav. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as well as through comparison with previously reported data. Two compounds were assessed for their inhibitory effect against pyruvate dehydrogenase E1α S300 phosphorylation. The fluorescent image suggested that both compounds (60 µM) revealed a stronger inhibition effect than the positive control (dichloroacetate, DCA 5 mM), with IC50 values of 99.82 µM and 80.25 µM, respectively.