Data in support for the measurement of serum 25-hydroxyvitamin D (25OHD) by tandem mass spectrometry
M.E. Jensen,
F.M. Ducharme,
Y. Théorêt,
A.-S. Bélanger,
E. Delvin
Affiliations
M.E. Jensen
Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences & School of Medicine and Public Health, University of Newcastle, Callaghan, Australia
F.M. Ducharme
Departments of Pediatrics and Social and Preventive Medicine, University of Montreal, Montreal, Canada; Clinical Research and Knowledge Transfer Unit, Research Centre, CHU Ste-Justine, Montreal, Canada
Y. Théorêt
Clinical Pharmacology Unit, Department of Clinical Biochemistry, CHU Ste-Justine, Canada; Department of Pharmacology, University of Montreal, Montreal, Canada
A.-S. Bélanger
Department of Clinical Biochemistry, CHU Ste-Justine, Canada
E. Delvin
Gatroenterology, Hepatology & Nutrition Division, CHU Ste-Justine Research Centre, University of Montreal, Montreal, Canada; Correspondence to: CHU Ste-Justine Research Centre, Gatroenterology, Hepatology & Nutrition Division, 3175 Côte Ste-Catherine, Montreal, QC, Canada H3T 1C5. Tel.: +1 514 345 4931x6268.
This article provides data and a method related to a research paper entitled “Assessing vitamin D nutritional status: is capillary blood adequate?” (Jensen et al., 2016) [1]. Circulating 25OHD, the accepted biomarker of the vitamin D nutritional status, is routinely measured by automated immunoassays, that although may be performed in hospital central laboratories, often suffer from a lack of specificity with regards to the different vitamin D metabolites, “Measurement of circulating 25-hydroxyvitamin D: a historical review” (Le Goff et al., 2015) [2]. Mass spectrometry offers this specificity. This article describes the performance of an in-house tandem mass spectrometry method for the individual measurement of 25OHD3, 25OHD2 and 3-épi-25OHD3. Keywords: Vitamin D, 25-hydroxyvitamin D, Mass spectrometry
