Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

David J. H. F. Knapp; Yale S. Michaels; Max Jamilly; Quentin R. V. Ferry; Hector Barbosa; Thomas A. Milne; Tudor A. Fulga

doi:10.1038/s41467-019-09148-3

Nature Communications (Apr 2019)

Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

David J. H. F. Knapp,
Yale S. Michaels,
Max Jamilly,
Quentin R. V. Ferry,
Hector Barbosa,
Thomas A. Milne,
Tudor A. Fulga

Affiliations

David J. H. F. Knapp: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Yale S. Michaels: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Max Jamilly: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Quentin R. V. Ferry: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Hector Barbosa: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford
Thomas A. Milne: Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford
Tudor A. Fulga: Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-019-09148-3
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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