A New Regimen for Induction of Metabolic Syndrome and the Effect of Febuxostat in Ovariectomized Female Albino Rats

Abstract

Background: Metabolic syndrome (MetS) is increasing worldwide and correlates with serum uric acid and high fat combined with highfructose diet (HFFD). Fat raises triglycerides. Fructose increases uric acid. We hypothesized that HFFD in menopause may have a pathogenic role in MetS and using febuxostat may improve it by attenuating oxidative stress and vascular inflammation. Objectives: This study aimed to assess effects of ovariectomy and HFFD diet on causing MetS for rats and effects of febuxostat on MetS of ovariectomized rats. Materials and methods: The study was carried on 50 female white albino rats, aged 3 months and weighted 170 to 300 gm. 50 rats were divided into: sham control, ovariectomized control,HFFD non-ovariectomized, HFFD ovariectomized and febuxostat HFFD ovariectomized group. HFFD was composed of 45% fat and 30% fructose for 12 weeks. The latter group received febuxostat for 8 weeks. Results: HFFD was associated with significant increased adiposity index (5.92±0.41), serum triglycerides (109.7±32.39 mg/dl), HOMA-IR (4.03±1.62) and serum uric acid (5.99 ±0.528 mg/dl). Ovariectomy has a synergistic effect with HFFD. HFFD with ovariectomy was also associated with significant increasedadiposity index (11.49±1.5),serum triglycerides (223.2±67.6 mg/dl), HOMA-IR (11.48±2.01) and serum uric acid (7.64±1.615mg/dl). Febuxostat was able to reverse these effects significantly in adiposity index (7.94±0.489), serum triglycerides (121.7±17.6 mg/dl) and serum uric acid (1.96±0.64mg/dl) but insignificant effect on HOMA-IR. Conclusions : Fructose and fat in menopause may have a major role in incidence of MetS. Febuxostat prevent pathological condition ofMetSby different mechanisms of action

Keywords

• metabolic syndrome; insulin resistance; hyperuricemia; fructose; febuxostat