Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats

Abstract

IntrodutionThe purpose of this study was investigating the effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats. Methods: 24 male Wistar rats were randomly assigned to three groups of eight, including diabetic group with endurance exercise (D-E), diabetic (D) and healthy control groups (Con). After induction of diabetic rats by injection streptozotocin was administered intraperitoneal , endurance exercise was applied for eight weeks, three sessions pre week in diabetic rats. Exercise intensity was equal to a speed of 21-25 m / min. The relative expression of CGI-58 and ATGL protein was measured with western blot technique and serum insulin and glucose levels were measured with a specialized kit. One-way ANOVA test was performed using SPSS-20 software and at a significance level less than 5%. Results: Results showed that ATGL and CGI-58 values were significantly different between the three groups (p <0.001). ATGL difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.001)was significant. CGI-58 difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.002) was significant. In addition, serum glucose and insulin levels decreased significantly after eight weeks of training (p <0.05). Conclusion: It seems that CGI-58 play a vital role in activating lipolysis by ATGL and increasing in CGI-58 leads to an increase in ATGL and ultimately leads to increased levels of intramuscular triglyceride oxidation and improved insulin resistance.

Keywords

• CGI-58
• ATGL
• endurance exercise
• insulin
• glucose