TRIAL TO CULTIVATE AND ISOLATE NEURONAL LIKE CELLS FROM 7 DAYS OLD MICE BRAIN

Abstract

In the present we know that each type of tissue possesses stem cells. These have the role to replace lost cells due to physiological turnover, injury, or disease and to support cell genesis contributing to the cell number homeostasis. Long time it was thought that adult mammalian central nervous system doesn't possess any or few regenerative capacity. Nowadays it was demonstrated that also in the brain there are stem cells which have the capacity to differentiate into astocytes, oligodendrocytes and neurons. In few degenerative diseases the stem cells lose the regenerative capacity with consequences in diminishing and loss of functional capacity. Stem cell therapy represents a novel and promising therapeutic approach to treatment of a variety of degenerative disease as multiple sclerosis. For this it is necessary that a efficient stem cell source can be found and secondary to be proven that these transplantable cells have differential potential into neural tissue. In order to be able to possess a stem cell source capable to build an implant it is necessary to know the cultivation technology and also the instruments to prove their capacity to differentiate into specific cells of the nervous system. These were the motives that enabled us to to try to harvest, cultivate and differentiate stem cells from the murine central nervous system.

Keywords

• neuronal stem cells
• neuronal like cells
• neurospheres