Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

Abstract

Summary: Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes.For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020).

Keywords

• Cell isolation
• Flow cytometry/mass cytometry
• Immunology