Effects of New Btk-Based Formulations BLB1 and Lip on Aquatic Non-Target Organisms
Sayda Dhaouadi,
Rim El Jeni,
Hazar Kraiem,
Gul Ayyildiz,
Cansu Filik-Iscen,
Zeynep Yurtkuran-Ceterez,
Balkiss Bouhaouala-Zahar
Affiliations
Sayda Dhaouadi
Laboratoire des Biomolécules, Venins et Applications Théranostiques, Equipe NanoBioMedika, Institut Pasteur de Tunis, Université Tunis-El Manar, 13 Place Pasteur, BP74, Belvédère, Tunis 1002, Tunisia
Rim El Jeni
Laboratoire des Biomolécules, Venins et Applications Théranostiques, Equipe NanoBioMedika, Institut Pasteur de Tunis, Université Tunis-El Manar, 13 Place Pasteur, BP74, Belvédère, Tunis 1002, Tunisia
Hazar Kraiem
Laboratoire des Biomolécules, Venins et Applications Théranostiques, Equipe NanoBioMedika, Institut Pasteur de Tunis, Université Tunis-El Manar, 13 Place Pasteur, BP74, Belvédère, Tunis 1002, Tunisia
Gul Ayyildiz
Biyans Biyolojik ÜRÜNLER AR-GE DAN. SAN. TİC.LTD.ŞTİ., Mustafa Kemal Mah. Dumlupinar BLV.NO: 280: G İÇ KAPI NO: 1260, Çankaya 06530, Turkey
Cansu Filik-Iscen
Department of Mathematics and Science Education, Faculty of Education, Eskisehir Osmangazi University, Eskisehir 26040, Turkey
Zeynep Yurtkuran-Ceterez
Biyans Biyolojik ÜRÜNLER AR-GE DAN. SAN. TİC.LTD.ŞTİ., Mustafa Kemal Mah. Dumlupinar BLV.NO: 280: G İÇ KAPI NO: 1260, Çankaya 06530, Turkey
Balkiss Bouhaouala-Zahar
Laboratoire des Biomolécules, Venins et Applications Théranostiques, Equipe NanoBioMedika, Institut Pasteur de Tunis, Université Tunis-El Manar, 13 Place Pasteur, BP74, Belvédère, Tunis 1002, Tunisia
Integrated pest management based on the use of biopesticides is largely applied. Experimental bioassays are critical to assess biopesticide biosafety at the ecotoxicological level. In this study, we investigated the effects of the new Bacillus thuringiensis subsp. kurstaki (Btk)-formulated-based biopesticides BLB1 and Lip, efficiently tested in field assays (IPM-4-CITRUS EC project no. 734921) on two aquatic non-target organisms, precisely the water flea Daphnia magna and the bioluminescent bacteria Aliivibrio fischeri. Acute toxicity studies, carried out in a comparative manner with Delfin® as the reference bioproduct and the lactose-based Blank formulation, show that no significant toxicity was observed up to 1 g/L. Our results indicated that BLB1- and Lip-formulated new bioproducts are far less toxic than the Delfin® reference bioproduct.
