Al Ameen Journal of Medical Sciences (Jan 2014)
Alteration of biochemical parameters after supplementation of multivitamins and minerals of sprayers on grape gardens of Western Maharashtra (India)
Abstract
Background: In the horticulture sector, the land used for cash crop like grapes is increasing particularly in Maharashtra state. There is an increasing use of the pesticides in an attempt to increase the yield and reduce the post harvest losses. The environmental pollution and poisoning due to wide use of pesticide during grape cultivation may be much more important factors to disturb the socio economical status of uneducated farm worker in rural areas. Aim and Objectives: The study aimed to evaluate the pesticide exposure of the population associated with grape cultivation with respect to biochemical effects, oxidative stress, antioxidants status and protecting them from such effects by supplementing vitamins A to Z. Material and Methods: For this study 30 subjects with occupational pesticides exposure i.e. sprayers of grape gardens having age in the range of 20 to 45 years from Tasgaon taluka, Sangli district, (Western Maharashtra) India were taken and all these study group subject,500 mg vitamin A to Z tablet / day for fifteen days were supplemented. The blood was collected by venipuncture from all these subjects for biochemical parameters assay before and after fifteen days of vitamins A to Z supplementation and all biochemical parameters were estimated by using standard methods. Results: Fifteen days vitamin A to Z and multi minerals supplementation to sprayers of grape gardens, we observed significant increase in serum Acetyl Cholinesterase (P<0.001, 2.32%), and decreased Aspartate Transaminase (P<0.05, 8.36%), Alanine Transaminase (P<0.05, 14.16%), serum Lipid Peroxide (P<0.001, 24.39%), Glutathione S-Transferase (P<0.01, 29.84%) and increased RBC-Superoxide Dismutase (P<0.05, 11.55%), RBC-Catalase (P<0.001, 25.7%), serum zinc (P<0.01, 3.74%), copper (P<0.001, 4.13%), blood lead (P<0.01, 3.33%) as compared to before vitamin A to Z and multi minerals supplementation and other biochemical parameters such as serum C-Reactive Proteins, Total Proteins, Albumin, Globulin, A/G ratio and Ceruloplasmin were not altered significantly as compared to before vitamin A to Z and multi minerals supplementation. Conclusions: Alteration of these biochemical parameters may be due to ameliorating effects of vitamin A to Z and multi minerals. The vitamins or minerals present in A to Z tablets may improve the hepatocellular damage caused by various pesticides, also beneficial for either decrease free radical formation or increased antioxidant status and increased AChE level may be decreased inhibition of AChE by pesticides of sprayers of grape gardens.
