Veterinaria (Mar 2017)

PHENOTYPIC AND GENOTYPIC ANALYSIS OF AN ARCANOBACTERIUM PLURANIMALIUM ISOLATED FROM A MUSKOX (OVIBOS MOSCHATUS)

  • Siti Gusti Ningrum,
  • Wyanda Arnafia,
  • Osama Sammra,
  • Mazen Alssahen,
  • Jörn-Peter Wickhorst,
  • Abdulwahed Ahmed Hassan,
  • Christoph Lämmler,
  • Ellen Prenger-Berninghoff,
  • Markus Timke,
  • Amir Abdulmawjood

Journal volume & issue
Vol. 66, no. 1
pp. 28 – 35

Abstract

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The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox (Ovibos moschatus) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf and the pluranimaliumlysin encoding gene pla allowed a successful identification of the isolated strain as A. pluranimalium. Gene pla could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of A. pluranimalium originated from a Muskox.