Dicliptera chinensis-derived polysaccharide enhanced the growth activity of submandibular gland cells in vitro after radiotherapy
Lixiang Zhao,
Yanchun Zhu,
Lihua Zhang,
Yude Huang,
Yiyang Fan,
Linjin Gao,
Yanfei Zhao,
Xian Wang,
Dongqing Mo,
Haoyu Lu,
Daiyou Wang
Affiliations
Lixiang Zhao
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Yanchun Zhu
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China; Xiaolan People's Hospital, ZhongShan, 528415, China
Lihua Zhang
Liuzhou People's Hospital, Liuzhou, 545000, China
Yude Huang
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Yiyang Fan
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China; Yichang City Hospital of Traditional Chinese Medicine, Yichang, 443000, China
Linjin Gao
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Yanfei Zhao
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Xian Wang
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Dongqing Mo
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Haoyu Lu
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China
Daiyou Wang
College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Deformity, Nanning, 530021, China; Corresponding author. College & Hospital of Stomatology, Guangxi Medical University, NO. 10 Shuangyong Road, Nanning, Guangxi, 530021, China.
Objective: Radiotherapy for head and neck can damage the salivary gland cells, which can easily result in xerostomia. No effective treatment for radiation-induced salivary gland dysfunction currently exists. Thus, we aimed to study the protective effect of Dicliptera chinensis polysaccharides (DCP) on the prevention of submandibular gland (SMG) cell damage caused by radiotherapy in Sprague-Dawley rats. Design: Mechanical enzyme digestion was used to extract primary rat SMG cells. A radiation injury model was established by treating these cells with a dose of 8 Gy, followed by intervention using different DCP concentrations. The cell counting kit 8 assay was used to determine the inhibition rate of SMG cells in each group. The rates of apoptosis and cell cycle progression were detected using flow cytometry. Expression of the Mre11/Rad50/Nbs1 complex (MRN) was detected using western blotting. Results: DCP increased the proliferation of SMG cells after irradiation, and cell growth activity positively correlated with polysaccharide concentration. Flow cytometry analysis of SMG cell apoptosis revealed that DCP markedly reduced the total apoptosis rate after irradiation, especially the early apoptosis rate. Cell cycle results suggested that DCP reduced the number of cells in the S and G2 phases after irradiation and alleviated the S and G2 blocks. Western blot results indicated that the expression of Mre11, Rad50, and Nbs1 decreased in the radiation-injured group, whereas their expression increased after DCP treatment. Conclusions: DCP can protect the rat SMG cells after radiation and be used as a protective agent against salivary gland cell damage caused by radiotherapy.
