Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics
Ye Tao,
Yannick Rossez,
Clovis Bortolus,
Luminita Duma,
Faustine Dubar,
Franck Merlier
Affiliations
Ye Tao
Sorbonne Universités, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire (GEC), UMR-CNRS 7025, CS 60319, 60203 Compiègne Cedex, France
Yannick Rossez
Sorbonne Universités, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire (GEC), UMR-CNRS 7025, CS 60319, 60203 Compiègne Cedex, France
Clovis Bortolus
INSERM U1285, Université de Lille, CHU de Lille, UMR CNRS 8576—UGSF—Unité de Glycobiologie Structurale et Fonctionnelle, 59000 Lille, France
Luminita Duma
Sorbonne Universités, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire (GEC), UMR-CNRS 7025, CS 60319, 60203 Compiègne Cedex, France
Faustine Dubar
INSERM U1285, Université de Lille, CHU de Lille, UMR CNRS 8576—UGSF—Unité de Glycobiologie Structurale et Fonctionnelle, 59000 Lille, France
Franck Merlier
Sorbonne Universités, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire (GEC), UMR-CNRS 7025, CS 60319, 60203 Compiègne Cedex, France
High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carry out metabolomic analyses as well as semi-quantification. In addition, the use of different clusters in negative mode makes it possible to compensate for deficiencies in linearity and inerrant saturation at time-of-flight detectors. The method has been approved and validated for different matrices, yeasts, and bacteria, and has shown differentiation between bacteria as a function of growth temperatures.
