Frontiers in Endocrinology (Apr 2022)

Thada Is Dispensable for Female Fertility in Mice

  • Shan Han,
  • Shan Han,
  • Yuqing Zhang,
  • Yuqing Zhang,
  • Yukun Zheng,
  • Yukun Zheng,
  • Congcong Liu,
  • Congcong Liu,
  • Yonghui Jiang,
  • Shigang Zhao,
  • Shigang Zhao,
  • Han Zhao,
  • Han Zhao

DOI
https://doi.org/10.3389/fendo.2022.787733
Journal volume & issue
Vol. 13

Abstract

Read online

Genome-wide association studies (GWAS) have identified THADA as one of the susceptibility genes for polycystic ovary syndrome (PCOS). Single nucleotide polymorphisms (SNPs) in the THADA gene showed significant over-transmission in PCOS and strong correlations with testosterone level. However, there was insufficient evidence to verify the effect of THADA in vivo on female reproductive system. In this study, we investigated the impacts of Thada ablation on ovarian function and reproductive outcomes with knockout (KO) mice. The results showed that the Thada deletion was insufficient to affect ovarian folliculogenesis, steroidogenesis, and female fertility. Additionally, we stressed the mice with high-fat-high-sugar diet (HFHS). In this case, the KO mice still merely had a negligible impact on ovarian function. These findings indicated that Thada deficiency was dispensable for female fertility in mice, which enriched our knowledge about in vivo functions of PCOS susceptibility genes.

Keywords