Nature Communications (Dec 2020)

A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

  • Sarah A. Nordeen,
  • Kasper R. Andersen,
  • Kevin E. Knockenhauer,
  • Jessica R. Ingram,
  • Hidde L. Ploegh,
  • Thomas U. Schwartz

DOI
https://doi.org/10.1038/s41467-020-19884-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.