Frontiers in Plant Science (Sep 2022)
HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley
- Xiuqin Feng,
- Xiuqin Feng,
- Md. Mostafijur Rahman,
- Md. Mostafijur Rahman,
- Qian Hu,
- Qian Hu,
- Bang Wang,
- Bang Wang,
- Hassan Karim,
- Hassan Karim,
- Carlos Guzmán,
- Wendy Harwood,
- Qiang Xu,
- Qiang Xu,
- Yazhou Zhang,
- Yazhou Zhang,
- Huaping Tang,
- Huaping Tang,
- Yunfeng Jiang,
- Yunfeng Jiang,
- Pengfei Qi,
- Pengfei Qi,
- Mei Deng,
- Mei Deng,
- Jian Ma,
- Jian Ma,
- Jingyu Lan,
- Jingyu Lan,
- Jirui Wang,
- Jirui Wang,
- Guoyue Chen,
- Guoyue Chen,
- Xiujin Lan,
- Xiujin Lan,
- Yuming Wei,
- Yuming Wei,
- Youliang Zheng,
- Youliang Zheng,
- Qiantao Jiang,
- Qiantao Jiang
Affiliations
- Xiuqin Feng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Xiuqin Feng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Md. Mostafijur Rahman
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Md. Mostafijur Rahman
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Qian Hu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Qian Hu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Bang Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Bang Wang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Hassan Karim
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Hassan Karim
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Carlos Guzmán
- Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Universidad de Córdoba, Cordoba, Spain
- Wendy Harwood
- John Innes Center, Norwich Research Park, Norwich, United Kingdom
- Qiang Xu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Qiang Xu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yazhou Zhang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Yazhou Zhang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Huaping Tang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Huaping Tang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yunfeng Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Yunfeng Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Pengfei Qi
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Pengfei Qi
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Mei Deng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Mei Deng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jian Ma
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Jian Ma
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jingyu Lan
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Jingyu Lan
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jirui Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Jirui Wang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Guoyue Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Guoyue Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xiujin Lan
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Xiujin Lan
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yuming Wei
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Yuming Wei
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Youliang Zheng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Youliang Zheng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Qiantao Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, SichuanChina
- Qiantao Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- DOI
- https://doi.org/10.3389/fpls.2022.1003333
- Journal volume & issue
-
Vol. 13
Abstract
Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3′ splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5′ of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism.
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