A pine leaf form acrylic resin coated coomassie brilliant blue g250-nido-carborane fluorescent complex strategy for tumor cell imaging

Abstract

Four distinct fluorescence complexes were prepared using a one-pot method: The L100-55-fluorescent complex (LFC), EPO-fluorescent complex (EFC), RS-fluorescent complex (SFC), and RL-fluorescent complex (RFC), respectively. The water solubility, selectivity, and visualization of the carborane have drawn a lot of attention. The carborane's low solubility is resolved, and Coomassie Brilliant Blue (CBB) G250-nido-carborane's biocompatibility is enhanced. The LFC complex was observed to scaly, branch and spread in (Scanning Electron Microscope) SEM and (Transmission Electron Microscope) TEM, like the pine leaves tightly covering the zwitteric complex. It was discovered that the fluorescent complex of LFC has strong selectivity and can accurately infiltrate tumor cells by imaging of tumor cells in vitro, suggesting that LFC has good biocompatibility. Human prostate cancer cells (PC3), human cervical cancer cells (HeLa) and Human normal liver cells (LO2) were evaluated under ten various concentration circumstances in the (Cell Counting Kit-8) CCK8 experiment. The proliferation impact was found to be 57.29% and 59.41%, respectively. shown the complex's strong anti-tumor action and little cytotoxicity. The design not only provides an excellent visual fluorescence targeting effect, but it also addresses the issue of nido-caborane-coomassie brilliant blue G250′s bioavailability.

Keywords

• Nido-caborane
• Coomassie brilliant blue G250
• Zwitteric complex
• Acrylic resin
• Tumor cell imaging