DNA PCR Assays for Igh Rearrangement

Yusuke  Satoh; Takao  Sudo; Takafumi  Yokota

doi:10.21769/BioProtoc.1046

Bio-Protocol (Feb 2014)

DNA PCR Assays for Igh Rearrangement

Yusuke Satoh,
Takao Sudo,
Takafumi Yokota

Affiliations

Yusuke Satoh: Department of Lifestyle Studies, Kobe Shoin Women’s University, Kobe, Japan
Takao Sudo: Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Suita, Japan
Takafumi Yokota: Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Suita, Japan

DOI: https://doi.org/10.21769/BioProtoc.1046
Journal volume & issue: Vol. 4, no. 4

Abstract

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This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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