Food and Agricultural Immunology (Jan 2018)

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

  • Liqiang Liu,
  • Liguang Xu,
  • Steven Suryoprabowo,
  • Shanshan Song,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1401043
Journal volume & issue
Vol. 29, no. 1
pp. 434 – 444

Abstract

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Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.

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