Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit
Brian Tenner,
Michael Getz,
Brian Ross,
Donya Ohadi,
Christopher H Bohrer,
Eric Greenwald,
Sohum Mehta,
Jie Xiao,
Padmini Rangamani,
Jin Zhang
Affiliations
Brian Tenner
Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine, Baltimore, United States; Department of Pharmacology, University of California, San Diego, La Jolla, United States
Chemical Engineering Graduate Program, University of California, San Diego, La Jolla, United States; Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, United States
Department of Pharmacology, University of California, San Diego, La Jolla, United States; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, United States
Signaling networks are spatiotemporally organized to sense diverse inputs, process information, and carry out specific cellular tasks. In β cells, Ca2+, cyclic adenosine monophosphate (cAMP), and Protein Kinase A (PKA) exist in an oscillatory circuit characterized by a high degree of feedback. Here, we describe a mode of regulation within this circuit involving a spatial dependence of the relative phase between cAMP, PKA, and Ca2+. We show that in mouse MIN6 β cells, nanodomain clustering of Ca2+-sensitive adenylyl cyclases (ACs) drives oscillations of local cAMP levels to be precisely in-phase with Ca2+ oscillations, whereas Ca2+-sensitive phosphodiesterases maintain out-of-phase oscillations outside of the nanodomain. Disruption of this precise phase relationship perturbs Ca2+ oscillations, suggesting the relative phase within an oscillatory circuit can encode specific functional information. This work unveils a novel mechanism of cAMP compartmentation utilized for localized tuning of an oscillatory circuit and has broad implications for the spatiotemporal regulation of signaling networks.
