Nature Communications (Aug 2020)
Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time
- Kayla G. Barnes,
- Anna E. Lachenauer,
- Adam Nitido,
- Sameed Siddiqui,
- Robin Gross,
- Brett Beitzel,
- Katherine J. Siddle,
- Catherine A. Freije,
- Bonnie Dighero-Kemp,
- Samar B. Mehta,
- Amber Carter,
- Jessica Uwanibe,
- Fehintola Ajogbasile,
- Testimony Olumade,
- Ikponmwosa Odia,
- John Demby Sandi,
- Mambu Momoh,
- Hayden C. Metsky,
- Chloe K. Boehm,
- Aaron E. Lin,
- Molly Kemball,
- Daniel J. Park,
- Luis Branco,
- Matt Boisen,
- Brian Sullivan,
- Mihret F. Amare,
- Abdulwasiu B. Tiamiyu,
- Zahra F. Parker,
- Michael Iroezindu,
- Donald S. Grant,
- Kayvon Modjarrad,
- Cameron Myhrvold,
- Robert F. Garry,
- Gustavo Palacios,
- Lisa E. Hensley,
- Stephen F. Schaffner,
- Christian T. Happi,
- Andres Colubri,
- Pardis C. Sabeti
Affiliations
- Kayla G. Barnes
- Broad Institute of MIT and Harvard
- Anna E. Lachenauer
- Broad Institute of MIT and Harvard
- Adam Nitido
- Ragon Institute of MGH, MIT, and Harvard
- Sameed Siddiqui
- Broad Institute of MIT and Harvard
- Robin Gross
- Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Brett Beitzel
- Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease
- Katherine J. Siddle
- Broad Institute of MIT and Harvard
- Catherine A. Freije
- Broad Institute of MIT and Harvard
- Bonnie Dighero-Kemp
- Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Samar B. Mehta
- Broad Institute of MIT and Harvard
- Amber Carter
- Broad Institute of MIT and Harvard
- Jessica Uwanibe
- African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer’s University
- Fehintola Ajogbasile
- African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer’s University
- Testimony Olumade
- African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer’s University
- Ikponmwosa Odia
- Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital
- John Demby Sandi
- African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer’s University
- Mambu Momoh
- African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer’s University
- Hayden C. Metsky
- Broad Institute of MIT and Harvard
- Chloe K. Boehm
- Broad Institute of MIT and Harvard
- Aaron E. Lin
- Broad Institute of MIT and Harvard
- Molly Kemball
- Broad Institute of MIT and Harvard
- Daniel J. Park
- Broad Institute of MIT and Harvard
- Luis Branco
- Zalgen Labs
- Matt Boisen
- Zalgen Labs
- Brian Sullivan
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Mihret F. Amare
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Abdulwasiu B. Tiamiyu
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Zahra F. Parker
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Michael Iroezindu
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Donald S. Grant
- Viral Hemorrhagic Fever Program, Kenema Government Hospital
- Kayvon Modjarrad
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Cameron Myhrvold
- Broad Institute of MIT and Harvard
- Robert F. Garry
- Zalgen Labs
- Gustavo Palacios
- Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease
- Lisa E. Hensley
- Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Stephen F. Schaffner
- Broad Institute of MIT and Harvard
- Christian T. Happi
- Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University
- Andres Colubri
- Broad Institute of MIT and Harvard
- Pardis C. Sabeti
- Broad Institute of MIT and Harvard
- DOI
- https://doi.org/10.1038/s41467-020-17994-9
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Outbreaks of viral hemorrhagic fevers highlight the need for sensitive, field-deployable diagnostics. Here the authors present a CRISPR-based SHERLOCK platform with field protocol and mobile app for Ebola and Lassa fever outbreaks.
