ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.

Keywords

• Educational garden
• Identification
• Morphology
• Yeast