Study the pathogenicity of Enterobacter cloacae in rats that isolated from diarrheatic buffalos calves in Babylon Province

Abstract

The study was aimed to isolate Enterobacter cloacae from feces of buffalo calves suffering from diarrhea and shows its pathogenicity in rats, 150 fecal samples were collected and cultured directly on MacConky agar then tested biochemically and with EPi 20 test to confirm a diagnosis of Enterobacter cloacae. After that injected 4 groups of rat with (106,107 and 108 CFU/ml) respectively, while the fourth group not treated and considered as a control group, also extracted the cell wall from Enterobacter and used four groups of rat to injected with different concentration (150, 250 and 350 μ/ml) of extracted cell wall respectively, while the fourth group considered as a control group. Results show that 10 isolates of Enterobacter were obtained from a stool and out of 10 isolates 7 isolates belong to Enterobacter cloacae. Bacterial isolation from internal organs shows the very heavy isolation of bacteria in dose 108 CFU/ml as compared with other doses, histopathological changes in organs (liver and spleen) of animals which injected with live bacteria and extracted cell wall reveal severe changes as compared with control groups.