Assessing Genetic Diversity and Population Differentiation in Wild Hop (<i>Humulus lupulus</i>) from the Region of Central Greece via SNP-NGS Genotyping
Konstantinos Tegopoulos,
Dimitrios V. Fountas,
Elisavet-Maria Andronidou,
Pantelis G. Bagos,
Petros Kolovos,
George Skavdis,
Panagiotis Pergantas,
Georgia G. Braliou,
Aristotelis C. Papageorgiou,
Maria E. Grigoriou
Affiliations
Konstantinos Tegopoulos
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
Dimitrios V. Fountas
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
Elisavet-Maria Andronidou
Department of Computer Science and Biomedical Informatics, University of Thessaly, 35100 Lamia, Greece
Pantelis G. Bagos
Department of Computer Science and Biomedical Informatics, University of Thessaly, 35100 Lamia, Greece
Petros Kolovos
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
George Skavdis
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
Panagiotis Pergantas
Bioapplications O.E., 32100 Livadia, Greece
Georgia G. Braliou
Department of Computer Science and Biomedical Informatics, University of Thessaly, 35100 Lamia, Greece
Aristotelis C. Papageorgiou
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
Maria E. Grigoriou
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
A growing need for the development of novel hop (Humulus lupulus) varieties has emerged as a result of the increasing demand for beers with distinct organoleptic characteristics and the expected impact of climate change on hop cultivars. As the genetic variation in the existing hop cultivars is low, wild hop germplasm can be used as a source for the development of novel cultivars. In this work, we analyzed, for the first time, the genetic diversity of H. lupulus var. lupulus wild germplasm in Greece. A SNP-NGS genotyping approach using a set of nine specific genetic markers, was employed in order to determine individual genotypes and to perform population structure analyses of wild hops from a region with complex topography, namely the Region of Central Greece. Our results revealed low differentiation among populations, with the spatial genetic patterns observed relating mainly to topographical elements rather than geographic distance. Interestingly, within wild hop populations, high genetic diversity was observed, showing that in the region of Central Greece, wild H. lupulus germplasm has significant potential that can be exploited in breeding programs towards the development of local, well adapted and potentially superior hop varieties.
