ADMET and DMPK (Mar 2016)

Reversed phase parallel artificial membrane permeation assay for log P measurement

  • Zihao Song,
  • Katsuhide Terada,
  • Kiyohiko Sugano

DOI
https://doi.org/10.5599/admet.4.1.277
Journal volume & issue
Vol. 4, no. 1
pp. 54 – 59

Abstract

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A reversed phase parallel artificial membrane permeation assay (RP-PAMPA) was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between log P and the apparent permeability was observed in the -0.24 < log P < 2.85 region (R2 = 0.98). RP-PAMPA was also applied to pKa measurement.