Quantitative characterization of the T cell receptor repertoires of human immunized by rabies virus vaccine

Abstract

Cellular immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the T cell receptor (TCR) repertoire in human after RABV vaccine immunization remained unclear. In this study, we conducted high-throughput sequencing of TCR β chain complementarity determining region 3(CDR3) repertoires in 4 healthy volunteers before and after immunization with RABV vaccine. Our data showed that RABV vaccination changed the TCR diversity and the usage of V/J gene segments, as well as V-J pairing. The high-frequency clonotypes that altered after vaccination were identified. These results may provide us with new insights into T cell receptor condition after RABV vaccination.

Keywords

• rabies virus (rabv)
• t cell receptor (tcr) repertoire
• high-throughput sequencing
• complementarity determining region 3(cdr3)
• immune response