Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters
Carmem Jane Ferreira Vilar,
Susana Barbosa Ribeiro,
Aurigena Antunes de Araújo,
Gerlane Coelho Bernardo Guerra,
Raimundo Fernandes de Araújo Júnior,
Gerly Anne de Castro Brito,
Renata Ferreira Carvalho Leitão,
Daniel de Lima Pontes,
Luiz Henrique Da Silva Gasparotto,
Maisie Mitchele Barbosa Oliveira,
Anderson Dias Viana,
Wendy Marina Toscano Queiroz de Medeiros,
Breno Gustavo Porfírio Bezerra,
Caroline Addison Carvalho Xavier de Medeiros
Affiliations
Carmem Jane Ferreira Vilar
Post Graduation Program in Biological Sciences, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Susana Barbosa Ribeiro
Post Graduation Program in Biotechnology-RENORBIO, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Aurigena Antunes de Araújo
Post Graduation Program in Dental Sciences/Post Graduation Program in Pharmaceutical Science/Department of Biophysics and Pharmacology, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Gerlane Coelho Bernardo Guerra
Post Graduation Program in Biological Sciences/Post Graduation Program in Pharmaceutical Science/Department of Biophysics and Pharmacology, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Raimundo Fernandes de Araújo Júnior
Post Graduation Program in Functional and Structural Biology/Post Graduation Program Health Science/Department of Morphology, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Gerly Anne de Castro Brito
Post Graduation Program in Morphofunctional Sciences/Department of Morphology, Faculty of Medicine, Federal University of Ceará, Rua Delmiro de Farias s/n, Rodolfo Teófilo, Fortaleza/CE 60416030, Brazil
Renata Ferreira Carvalho Leitão
Post Graduation Program in Morphofunctional Sciences/Department of Morphology, Faculty of Medicine, Federal University of Ceará, Rua Delmiro de Farias s/n, Rodolfo Teófilo, Fortaleza/CE 60416030, Brazil
Daniel de Lima Pontes
Post Graduation Program of Chemistry/Institute of Chemistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Luiz Henrique Da Silva Gasparotto
Post Graduation Program of Chemistry/Institute of Chemistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Maisie Mitchele Barbosa Oliveira
Post Graduation Program in Biotechnology-RENORBIO, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Anderson Dias Viana
Post Graduation Program of Chemistry/Institute of Chemistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Wendy Marina Toscano Queiroz de Medeiros
Post Graduation Program of Chemistry/Institute of Chemistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Breno Gustavo Porfírio Bezerra
Post Graduation Program of Chemistry/Institute of Chemistry, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Caroline Addison Carvalho Xavier de Medeiros
Post Graduation Program in Biological Sciences, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho 3000, Lagoa Nova, Natal/RN 59078970, Brazil
Background: Oral mucositis (OM) is a severe inflammation of the oral mucosal cells associated with chemotherapy and/or radiotherapy-induced toxicity, resulting in epithelial ulcers and higher risk of death from sepsis. The aim of the present study was to evaluate the nanoparticle (AuNp) effect on OM induced in hamsters. Materials and methods: 5-fluorouracil (5FU) was used on the first and second day of the experimental model in Golden sirian hamsters, and on the fourth day, mechanical trauma was applied to induce OM. The animals were divided into groups, i.e., polyvinylpyrrolidone (PVP), mechanical trauma (MT), 5FU, and groups treated with gold nanoparticles (AuNps) (62.5, 125, and 250 μg/kg). On the 10th day, animals were euthanized for macroscopic, histopathological, immunohistochemical, western blot, quantitative polymerase chain reaction (qRT-PCR), and AuNp quantification. Results: AuNp (250 μg/kg) reduced TNF-α, IL-1β, COX-2, NF-κB, TGF-β, and SMAD 2/3; increased glutathione levels; decreased the expression of Kelch ECH-associated protein 1 (KEAP1); and induced heme oxygenase 1 (HMOX-1) and NAD (P) H quinone oxidoreductase 1 (NQO1) genes. Conclusions: AuNp (250 μg/kg) prevented 5-FU-induced OM in hamsters and improved the parameters of inflammation and oxidative stress.
