Scientific Data (Jul 2024)

A chromosome-level genome assembly and evolutionary analysis of Coregonus ussuriensis Berg

  • Tianqing Huang,
  • Enhui Liu,
  • Baorui Cao,
  • Wenwen Li,
  • Gaochao Wang,
  • Wei Gu,
  • Haibing Ma,
  • Fulin Dong,
  • Bingqian Wang,
  • Gefeng Xu

DOI
https://doi.org/10.1038/s41597-024-03642-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

Read online

Abstract Coregonus ussuriensis Berg, distributed widely in cold waters above 45° N latitude, is a savored freshwater whitefish that has been included in the list of endangered animals as a consequence of overfishing. Lack of genomic information seriously hampers evolutionary and genetic research on C. ussuriensis warranting the need to assemble a high-quality reference genome to promote its genetic breeding. We assembled and constructed a reference chromosome-level C. ussuriensis genome (sequence length, 2.51 Gb; contig N50 length, 4.27 Mb) using PacBio sequencing and Hi-C assembly technology, 3,109 contigs were assembled into scaffolds, resulting in a genome assembly with 40 chromosomes and a scaffold N50 length of 62.20 Mb. In addition, 43,320 protein-coding genes were annotated. The peak Ks position in the species comparison reflects the whole-genome replication event of C. ussuriensis. This chromosome-level genome provides reference data for further studies on the molecular breeding of C. ussuriensis.