Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia

Sean K. Ryan; Kelly L. Jordan-Sciutto; Stewart A. Anderson

STAR Protocols (Dec 2020)

Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia

Sean K. Ryan,
Kelly L. Jordan-Sciutto,
Stewart A. Anderson

Affiliations

Sean K. Ryan: Department of Pathology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Psychiatry, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
Kelly L. Jordan-Sciutto: Department of Pathology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Stewart A. Anderson: Department of Psychiatry, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 3
p. 100190

Abstract

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Summary: This protocol establishes a tri-culture of hiPSC-derived neurons, astrocytes, and microglia for the study of cellular interactions during homeostasis, injury, and disease. This system allows for mechanistic studies that can identify the roles of individual cell types in disease and injury response in a physiologically relevant, all-human system. This protocol utilizes and modifies prior differentiations. Limitations include the prolonged maturation of human astrocytes and neurons and scalability.For complete details on the use and execution of this protocol, please refer to Ryan et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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