Oxyresveratrol modulates the immune response in vitro
Palabiyik-Yucelik Saziye Sezin,
Moser Simone,
Becker Kathrin,
Halici Zekai,
Bayir Yasin,
Stonig Marlies,
Schennach Harald,
Fuchs Dietmar,
Gostner Johanna M.,
Kurz Katharina
Affiliations
Palabiyik-Yucelik Saziye Sezin
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey
Moser Simone
Department of Pharmacy, Pharmaceutical Biology, Ludwig-Maximilians University of Munich, Munich, Germany
Becker Kathrin
Institute of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Halici Zekai
Clinical Research, Development and Design Application and Research Center, Atatürk University, Erzurum, Turkey
Bayir Yasin
Department of Biochemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey
Stonig Marlies
Institute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Schennach Harald
Central Institute of Blood Transfusion and Immunology, University Hospital of Innsbruck, Innsbruck, Austria
Fuchs Dietmar
Institute of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Gostner Johanna M.
Institute of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Kurz Katharina
Department of Internal Medicine II, Infectious Diseases, Pneumology, Rheumatology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria
The naturally occurring stilbenoid oxyresveratrol was shown to influence inflammatory and metabolic processes. During cellular immune activation, tryptophan breakdown and neopterin formation via the enzymes indoleamine 2,3-dioxygenase-1 (IDO-1) and GTP-cyclohydrolase, respectively, are induced. Neopterin and the kynurenine to tryptophan ratio are reliable and pertinent biomarkers of Th1-type immune response and are also used in vitro to monitor effects of active plant ingredients on peripheral blood mononuclear cells (PBMCs). We investigated the effects of oxyresveratrol on the activity of the above-mentioned pathways in mitogen-stimulated human PBMC and in the myelomonocytic cell line THP-1. Oxyresveratrol exerted suppressive effects on tryptophan breakdown in both stimulated cell models. Of note, in PBMC, tryptophan breakdown was induced at lower concentrations (5–20 µM) and suppressed at higher treatment concentrations only. Neopterin formation was decreased dose-dependently in stimulated PBMC. In unstimulated PBMC similar, albeit lesser effects were observed. Data indicate that oxyresveratrol exerts distinct and concentration-dependent effects on different immune cell types. IDO-1 is targeted by oxyresveratrol and its activity can be modulated in both directions. Detailed investigations of the interactions would be interesting to fully explore the activity of this phytocompound.
