Nature Communications (Sep 2019)
Molecular basis of microhomology-mediated end-joining by purified full-length Polθ
- Samuel J. Black,
- Ahmet Y. Ozdemir,
- Ekaterina Kashkina,
- Tatiana Kent,
- Timur Rusanov,
- Dejan Ristic,
- Yeonoh Shin,
- Antonio Suma,
- Trung Hoang,
- Gurushankar Chandramouly,
- Labiba A. Siddique,
- Nikita Borisonnik,
- Katherine Sullivan-Reed,
- Joseph S. Mallon,
- Tomasz Skorski,
- Vincenzo Carnevale,
- Katsuhiko S. Murakami,
- Claire Wyman,
- Richard T. Pomerantz
Affiliations
- Samuel J. Black
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Ahmet Y. Ozdemir
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Ekaterina Kashkina
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Tatiana Kent
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Timur Rusanov
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Dejan Ristic
- Department of Molecular Genetics and Department of Radiation Oncology, Erasmus University Medical Center
- Yeonoh Shin
- Department of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, Pennsylvania State University
- Antonio Suma
- Institute for Computational Molecular Science, Temple University
- Trung Hoang
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Gurushankar Chandramouly
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Labiba A. Siddique
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Nikita Borisonnik
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Katherine Sullivan-Reed
- Fels Institute for Cancer Research, Department of Microbiology and Immunology, Temple University Lewis Katz School of Medicine
- Joseph S. Mallon
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- Tomasz Skorski
- Fels Institute for Cancer Research, Department of Microbiology and Immunology, Temple University Lewis Katz School of Medicine
- Vincenzo Carnevale
- Institute for Computational Molecular Science, Temple University
- Katsuhiko S. Murakami
- Department of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, Pennsylvania State University
- Claire Wyman
- Department of Molecular Genetics and Department of Radiation Oncology, Erasmus University Medical Center
- Richard T. Pomerantz
- Fels Institute for Cancer Research, Department of Medical Genetics and Molecular Biochemistry, Temple University Lewis Katz School of Medicine
- DOI
- https://doi.org/10.1038/s41467-019-12272-9
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 16
Abstract
DNA polymerase θ is a polymerase-helicase essential for microhomology-mediated end-joining (MMEJ) or alternative end-joining of DNA. Here the authors use biochemical and biophysical methods to reveal how full-length human DNA polymerase θ performs MMEJ at the molecular level.
