An artificialed protein corona coating the surface of magnetic nanoparicles:a simple and efficient method for label antibody
Penghua Zhao,
Xiaoyan Huang,
Yaping Li,
Xueping Huo,
Qing Feng,
Xiangrong Zhao,
Cuixiang Xu,
Jianhua Wang
Affiliations
Penghua Zhao
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Xiaoyan Huang
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Yaping Li
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Xueping Huo
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Qing Feng
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Xiangrong Zhao
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China
Cuixiang Xu
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Corresponding author. Shaanxi Provincial People's Hospital, 256 West Youyi Road, Xi'an, 710068, China.
Jianhua Wang
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, 710068, China; Corresponding author. Shaanxi Provincial People's Hospital, 256 West Youyi Road, Xi'an, 710068, China.
Background: Protein Corona (PC) of nanoparticles is a structure which composed of one or more layers of proteins adsorbed on the surface of nanomaterials, and the formation of PC is a universal process of spontaneous randomness. We take advantage of the formation principle of the PC, developed a simple and efficient method for label protein to nanoparticles. Methods: The artificialed protein corona (APC) on the surface of nanoparticles was synthesized via the artificialed methods of desolvation aggregation and crosslinking with control. Results: The dosage of precipitator and the ratio of protein to magnetic nanoparticles (MNPs)(particle size: 3 nm) were optimized, and the core-shell nanoparticles with narrow particle size (particle size: 10 nm) distribution were obtained. The MNPs with APC were characterized by transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Additionally, a hemolysis test on prepared MNPs was conducted with APC. The presence of APC coating on the surface of MNPs showed an improving effect to reduce the cytotoxicity. Cellular toxicity of MNPs with APC was also investigated on HFF1 cell lines. And the cells survival in the presence of APC coated MNPs and display neither reduced metabolism nor cytostatic effect. The functional test of the MNPs with APC showed that proteins can be modified and labeled onto magnetic nanoparticles and retain their original activity. Conclusions: This marking method is gentle and effective. And the properties of the APC propose MNPs as a promising candidate for multifunctional biomedical applications.
