Assessment of In Vivo Antioxidant Activity of Anogeissus latifolia Bark in Ethanol-induced Oxidative Stress in Rats

Abstract

Substances with hepatoprotective activity can inhibit oxidation to protect the body's cells from the damaging effects of oxidation. It can bind to free oxygen radicals preventing these radicals from damaging healthy cells. The present investigation aims to evaluate the in vivo antioxidant potential of the bark of Anogeissus latifolia in ethanol-induced hepatotoxicity. Animals were treated with the methanol extract of A. latifolia (MEAL) for 15 days and induced oxidative stress with a single dose of ethanol (36 mg/kg po). The activity was measured by measuring oxidative stress markers such as lipid peroxidation, reduced glutathione, superoxide dismutase, and catalase levels in hepatotoxic rats. Administration of MEAL at 100 and 200 mg/kgBW marked decreased ethanol-induced elevation levels of oxidative stress markers and liver in a dose-dependent manner. The effects of the extract were compared with the Silymarin standard at 100 mg/kgBW. In methanol extract-treated animals, the toxic effect of ethanol was controlled significantly (P <0.05) by restoration of the levels of enzymes as compared to the normal and standard groups. The results concluded that the MEAL bark possesses significant in vivo antioxidant activity and can be exploited in protecting hepatic tissue from oxidative stress.

Keywords

• Anogeissus latifolia
• ethanol
• oxidative stress markers
• in vivo antioxidant activity