Immunological comparison of the NADH:nitrate reductase from different cucumber tissues

Abstract

Soluble nitrate reductase from cucumber roots (Cucumis sativus L.) was isolated and purified with blue-Sepharose 4B. Specific antibodies against the NR protein were raised by immunization of a goat. Using polyclonal antibodies anti-NR properties of the nitrate reductase from various cucumber tissues were examined. Experiments showed difference in immuno-logical properties of nitrate reductase (NR) from cotyledon roots and leaves.

Keywords

• nitrate reductase
• antibody
• dot-blot
• immunological assays
• Cucumis sativus