Centro Militar de Cría Caballar de Ávila (CCFAA), C/Arsenio Gutiérrez Palacios s/n, 05005 Ávila, Spain
Carmen Galán
Animal Medicine and Surgery Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain
Mónica Domínguez-Gimbernat
Animal Medicine and Surgery Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain
Agustín Oliet
Animal Selection and Reproduction Center, Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA), Ctra. Colmenar Viejo a Guadalix de la Sierra, km 1, Colmenar Viejo, 28770 Madrid, Spain
Santiago Moreno
Animal Selection and Reproduction Center, Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA), Ctra. Colmenar Viejo a Guadalix de la Sierra, km 1, Colmenar Viejo, 28770 Madrid, Spain
Bruno González-Zorn
Animal Health Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain
Luna Gutiérrez-Cepeda
Animal Medicine and Surgery Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain
The study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing and after 48 h of refrigeration. The aim was to explore the potential reduction of prophylactic antibiotic usage in semen extenders. Semen from ten adult stallions was collected and processed under a strict hygiene protocol and divided into four aliquots: Simple Centrifugation with antibiotics (SC+), Simple Centrifugation (SC−), Single-Layer Colloidal Centrifugation (CC−), and Filtration (with SpermFilter®) (F−), all in extenders without antibiotics. Sperm motility, viability, and microbial load on three culture media were assessed. No significant differences were observed in the main in the sperm quality parameters among the four protocols post-processing and at 48 h (p p p p p < 0.10) were found in 48 h chilled samples. Regardless of antibiotic presence, the evaluated processing methods, when combined with rigorous hygiene measures, maintained semen quality and reduced microbial load to the same extent as a traditional protocol using antibiotics.
