Anticonvulsant Effects of Pasipay (Prepared from Passion Flower) by PTZ Model in the Mice

Abstract

Background: Passion flower has shown antianxeity and sedative effects in human. Objective: In this study, anticonvulsant effects of hydroalcoholic extract of passiflora were studied. Methods: Anticonvulsant effects of Pasipay were examined by using pentylenetetrazole model (PTZ) on mice. In this research pasipay, diazepam, and normal saline were injected i.p. at doses (0.05-0.4 mg/kg), (0.5-1 mg/kg), (10 ml/kg) respectively and after 30 minutes, PTZ (90 mg/kg, i.p) was injected to these mice. For investigating the mechanism of pasipay, flumazenil (2 mg/kg, i.p) and naloxone (5 mg/kg, i.p) were also injected 5 minutes before pasipay. Results: An ED50 value of pasipay was 0.2 mg/kg (%95 CL: 0.119, 0.408). In this model, pasipay at the dose of 0.4 mg/kg prolonged the onset time of seizure and decreased the duration of seizures compared to control (p<0.001). In this dose the seizure and mortality protection was 100%. Flumazenil and naloxone could suppress anticonvulsant effects of pasipay. Conclusion: It seems that pasipay may be useful for treatment of absence seizure and these effects may be related to the effect of it on GABAergic and opioid systems.

Keywords

• passion flower
• pasipay
• anticonvulsant effects
• pentylenetetrazol