Serological and Molecular Analysis of the Results of Medical Staff Vaccination Against Hepatitis

Abstract

The results of the immune response intensity and duration in 890 hospital staff after vaccination against hepatitis B are presented. High frequency of HBsAg (1,7%), anti-HBs (21,1%), anti-HВe (16,9%) among not vaccinated against hepatitis B medical staff in comparison with vaccinated persons (0,2, 10,7 and 6,4% respectively) was established. «Isolated» anti-HBc was estimated to occur at 5 (0,8%) vaccinated and 15 (6,3%) not vaccinated persons, HBV DNA — at 3 (0,5%) and 5 (2,1%), respectively. Among 653 vaccinated medical staff antiHBs were absent or their concentration was below protective level at 140 (21,4%) persons. HBV DNA positive in PCR isolates had genotype D, subtypes ayw3 and ayw2. Sequence analysis of PCR products amplified from the S region revealed site replacements T118A, T118V, M125T, A128V. The necessity of the new generation vaccines formation capable to protect from HBV «wild» strains and mutant forms is discussed.

Keywords

• hepatitis b virus
• medical staff
• vaccination against hepatitis b
• vaccine-induced immunity
• hb-mutants