Frontiers in Cellular and Infection Microbiology (Nov 2021)

Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts

  • Tao Dong,
  • Tao Dong,
  • Weiwei Wang,
  • Weiwei Wang,
  • Minhao Xia,
  • Minhao Xia,
  • Shujie Liang,
  • Shujie Liang,
  • Guangzhong Hu,
  • Guangzhong Hu,
  • Hui Ye,
  • Hui Ye,
  • Qingyun Cao,
  • Qingyun Cao,
  • Zemin Dong,
  • Zemin Dong,
  • Changming Zhang,
  • Changming Zhang,
  • Dingyuan Feng,
  • Dingyuan Feng,
  • Jianjun Zuo,
  • Jianjun Zuo

DOI
https://doi.org/10.3389/fcimb.2021.758898
Journal volume & issue
Vol. 11

Abstract

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Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress. However, the potential association of HtpG with S. Typhimurium infection remains unknown. In this study, we clarified that HtpG could also play a role as an effector in S. Typhimurium infection. RNA-seq indicated that the flagellar assembly pathway, infection pathway, and chemotaxis pathway genes of S. Typhimurium were downregulated after the mutation of HtpG, which resulted in compromises of S. Typhimurium motility, biofilm formation, adhesion, invasion, and inflammation-inducing ability. In addition, HtpG recombinant protein was capable of promoting the proliferation of S. Typhimurium in host cells and the resultant inflammation. Collectively, our results illustrated an important role of HtpG in S. Typhimurium infection.

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