Characterization of Tomato Brown Rugose Fruit Virus (ToBRFV) Detected in Czech Republic
Ales Eichmeier,
Miroslava Hejlova,
Hana Orsagova,
Lucie Frejlichova,
Eliska Hakalova,
Katerina Tomankova,
Sarka Linhartova,
Pavel Kulich,
Vaclav Cermak,
Jana Cechova
Affiliations
Ales Eichmeier
Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic
Miroslava Hejlova
Division of Plant Pests Diagnostics, National Reference Laboratory, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic
Hana Orsagova
Division of Plant Pests Diagnostics, National Reference Laboratory, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic
Lucie Frejlichova
Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic
Eliska Hakalova
Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic
Katerina Tomankova
Division of Plant Pests Diagnostics, National Reference Laboratory, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic
Sarka Linhartova
Division of Plant Pests Diagnostics, National Reference Laboratory, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic
Pavel Kulich
Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
Vaclav Cermak
Division of Plant Pests Diagnostics, National Reference Laboratory, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic
Jana Cechova
Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic
Tomato is the most consumed vegetable in the world. The tomato brown rugose fruit virus (ToBRFV) is an important destructive virus that damages tomatoes and peppers with significant economic impact. The detection and characterization of this important viral pathogen were evaluated at the molecular and morphological level. The viral isolate was purified and inoculated on tomato and pepper plants. Small RNAs were sequenced in both plants and the profiles were compared. The complete genome of the isolate was obtained, and microRNA (miRNA) profiles were unveiled by small RNA sequencing. Symptoms caused by the isolate were also described and the morphology of the isolate was observed by transmission electron microscopy. Our results contribute to further understanding of the role of miRNAs in ToBRFV pathogenesis, which may be crucial for understanding disease symptom development in tomatoes and peppers.
