Nature Communications (Jun 2019)
Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics
- Anja Greule,
- Thierry Izoré,
- Dumitrita Iftime,
- Julien Tailhades,
- Melanie Schoppet,
- Yongwei Zhao,
- Madeleine Peschke,
- Iftekhar Ahmed,
- Andreas Kulik,
- Martina Adamek,
- Robert J. A. Goode,
- Ralf B. Schittenhelm,
- Joe A. Kaczmarski,
- Colin J. Jackson,
- Nadine Ziemert,
- Elizabeth H. Krenske,
- James J. De Voss,
- Evi Stegmann,
- Max J. Cryle
Affiliations
- Anja Greule
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Thierry Izoré
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Dumitrita Iftime
- Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/Biotechnology, University of Tübingen
- Julien Tailhades
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Melanie Schoppet
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Yongwei Zhao
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Madeleine Peschke
- Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research
- Iftekhar Ahmed
- Department of Chemistry, The University of Queensland
- Andreas Kulik
- Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/Biotechnology, University of Tübingen
- Martina Adamek
- Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/Biotechnology, University of Tübingen
- Robert J. A. Goode
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Ralf B. Schittenhelm
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- Joe A. Kaczmarski
- Research School of Chemistry, The Australian National University
- Colin J. Jackson
- Research School of Chemistry, The Australian National University
- Nadine Ziemert
- Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/Biotechnology, University of Tübingen
- Elizabeth H. Krenske
- Department of Chemistry, The University of Queensland
- James J. De Voss
- Department of Chemistry, The University of Queensland
- Evi Stegmann
- Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Microbiology/Biotechnology, University of Tübingen
- Max J. Cryle
- Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University
- DOI
- https://doi.org/10.1038/s41467-019-10384-w
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 15
Abstract
Kistamicin is a structurally divergent glycopeptide antibiotic (GPA) that contains a unique 15-membered A-O-B ring. Here, the authors obtained a crystal structure of the kistamicin OxyA/X-domain complex and analysed the cyclisation cascade leading to the formation of the A-O-B ring.
