Nature Communications (Nov 2017)
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate
- Takehiko Tosha,
- Takashi Nomura,
- Takuma Nishida,
- Naoya Saeki,
- Kouta Okubayashi,
- Raika Yamagiwa,
- Michihiro Sugahara,
- Takanori Nakane,
- Keitaro Yamashita,
- Kunio Hirata,
- Go Ueno,
- Tetsunari Kimura,
- Tamao Hisano,
- Kazumasa Muramoto,
- Hitomi Sawai,
- Hanae Takeda,
- Eiichi Mizohata,
- Ayumi Yamashita,
- Yusuke Kanematsu,
- Yu Takano,
- Eriko Nango,
- Rie Tanaka,
- Osamu Nureki,
- Osami Shoji,
- Yuka Ikemoto,
- Hironori Murakami,
- Shigeki Owada,
- Kensuke Tono,
- Makina Yabashi,
- Masaki Yamamoto,
- Hideo Ago,
- So Iwata,
- Hiroshi Sugimoto,
- Yoshitsugu Shiro,
- Minoru Kubo
Affiliations
- Takehiko Tosha
- RIKEN SPring-8 Center
- Takashi Nomura
- RIKEN SPring-8 Center
- Takuma Nishida
- Department of Life Science, Graduate School of Life Science, University of Hyogo
- Naoya Saeki
- Department of Life Science, Graduate School of Life Science, University of Hyogo
- Kouta Okubayashi
- Department of Life Science, Graduate School of Life Science, University of Hyogo
- Raika Yamagiwa
- RIKEN SPring-8 Center
- Michihiro Sugahara
- RIKEN SPring-8 Center
- Takanori Nakane
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- Keitaro Yamashita
- RIKEN SPring-8 Center
- Kunio Hirata
- RIKEN SPring-8 Center
- Go Ueno
- RIKEN SPring-8 Center
- Tetsunari Kimura
- Department of Chemistry, Graduate School of Science, Kobe University
- Tamao Hisano
- RIKEN SPring-8 Center
- Kazumasa Muramoto
- Department of Life Science, Graduate School of Life Science, University of Hyogo
- Hitomi Sawai
- Department of Life Science, Graduate School of Life Science, University of Hyogo
- Hanae Takeda
- RIKEN SPring-8 Center
- Eiichi Mizohata
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University
- Ayumi Yamashita
- RIKEN SPring-8 Center
- Yusuke Kanematsu
- Graduate School of Information Sciences, Hiroshima City University
- Yu Takano
- Graduate School of Information Sciences, Hiroshima City University
- Eriko Nango
- RIKEN SPring-8 Center
- Rie Tanaka
- RIKEN SPring-8 Center
- Osamu Nureki
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- Osami Shoji
- Department of Chemistry, Graduate School of Science, Nagoya University
- Yuka Ikemoto
- Japan Synchrotron Radiation Research Institute
- Hironori Murakami
- Japan Synchrotron Radiation Research Institute
- Shigeki Owada
- RIKEN SPring-8 Center
- Kensuke Tono
- Japan Synchrotron Radiation Research Institute
- Makina Yabashi
- RIKEN SPring-8 Center
- Masaki Yamamoto
- RIKEN SPring-8 Center
- Hideo Ago
- RIKEN SPring-8 Center
- So Iwata
- RIKEN SPring-8 Center
- Hiroshi Sugimoto
- RIKEN SPring-8 Center
- Yoshitsugu Shiro
- RIKEN SPring-8 Center
- Minoru Kubo
- RIKEN SPring-8 Center
- DOI
- https://doi.org/10.1038/s41467-017-01702-1
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 9
Abstract
Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase.
