INVESTIGATION OF CUCUMBER MOSAIC VIRUS INFECTION BY SEROLOGICAL AND MOLECULAR METHODS ON ORNAMENTAL BULBOUS PLANTS IN ÇANAKKALE PROVINCE AND ITS DISTRICTS

Abstract

There are many problems during cultivation of ornamental plants arising from diseases and pests. Viral borne pathogens, among these diseases, have a distinctive importance compared to other plant diseases due to their easy mechanical transmission from plant to plant, their rapid spreading ability by means of seeds, vegetative material and vector insects and the absence of a chemical control method. Cucumber mosaic virus (CMV) is one of these virus diseases. CMV is the second virus after tobacco mosaic virus (TMV) over the world in terms of the number of hosts. In this study, presence of CMV was investigated in ornamental bulbous plants (daffodils, hyacinths, lilies) in Çanakkale province and its districts. For this purpose, 128 bulbous ornamental plant samples showing virus and virus like disease symptoms were collected in field studies performed between the years 2012-2015. The collected samples were tested for the presence of CMV using serological and molecular methods with DAS-ELISA and RT-PCR, respectively. Twenty-nine out of 128 samples (%22.65) were found to be infected with CMV. Chlorosis was observed throughout veins and light mosaics were observed to be the most common symptoms in CMV infected plants.

Keywords

• cmv
• süs bitkileri
• das-elisa
• rt-pcr
• cmv
• ornamental plants
• das-elisa
• rt-pcr